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KMID : 0043319930160020155
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Archives of Pharmacal Research
1993 Volume.16 No. 2 p.155 ~ p.157
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DNA Transfection in SK-N-BE(2)C Human Neuroblastoma Cells
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Lee Myung-Koo
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Abstract
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DNA transfection conditions were investigated by calcium phosphate-DNA co-precipitation in SK-N-BE(2)C human neuroblastoma cells. The DNA plasmid of TH2400CAT was used in which rat tyrosine hydroxylase gene was inserted into chloramphenicol acetyltransferase reporter gent. The transfection efficiency was 25-30% and the method was simple and reproducible. So, the method will be a good tool for transient transfection analysis.
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KEYWORD
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Transfection analysis, Human neuroblastrma cell, Tyrosine hyroxylase, Chloram-phenicol acetyltrasferae assay
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